Product•Sequencing Grade Enzymes

SG-rTrypsin(Liquid)
 
Sequencing Grade Modified Trypsin

 
Catalog Number:SRT0202
CAS:9002-07-7
EC:3.4.21.4
Storage temperature: -70℃
Source: Sequencing grade modified trypsin is a genetically engineered methylation modified protein expressed in E.coli 
Protein Sequence:The amino acid sequence of sequencing grade modified trypsin is identical to porcine pancreas-derived trypsin
 
1.DESCRIPTION
YaxinBio sequencing grade trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant  trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.
 
2.MAIN FEATURES

Source Recombinant E. coli
Appearance Clear, colorless liquid
Specific activity ≥4500 USP units/mg pro.
Protein concentrate 0.5mg/ml in 50mM HAC
SDS-PAGE Single major band
Molecular Weight(SDS-PAGE) 24.0±2.4 kDa
Purity(HPLC) ≥95%
 
3.RECOMMEND USAGE
1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7.0-8.0 buffers directly. 1mM CaClis recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7.0-8.0. 
2. When using this product, thaw it at room temperature, and mix gently before use.
 
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: 1.The solution should be stored under -70℃, It is stable within 24 months.
2.Above 90% activity is remained after kept under 4℃ or 25℃ for 24h.
3.No activity loss after 5 times repeated freezing and thawing.
4. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin in 50mM NH4HCOis retained above 95% after a 3 hours incubation at 37 ℃ . For long-term such as 20 hours incubation, 1mM CaCl2 is recommended to be contained.
Stability of transport: The product is stable by blue ice insulation transport.
 
5.PRODUCT ADVANTAGE
Sequence grade recombinant protein: high specificity,good stability.
Animal origin free: Sequencing grade modified trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
 
6.PRODUCT USAGE
The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.
 
7.RELATED PRODUCTS
Recombinant enterokinase
Recombinant V8 protease
Sequencing grade chymotrypsin
Sequencing grade carboxypeptidase B

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