Product•Recombinant Enzymes

R-Soybean Trypsin Inhibitor
Recombinant Soybean Trypsin Inhibitor
 
Catalog No.: RSTI19
CAS: 9035-81-8
Synonyms: Soybean Trypsin Inhibitor,Kunitz Trypsin Inhibitor,Tia1,STI,SBT1
Source: Recombinant soybean trypsin inhibitor is a genetically engineered protein expressed in E.coli 
Storage temperature: 2-8℃,sealed,dark
 
1.DESCRIPTION
Recombinant Soybean Trypsin Inhibitor is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. The ability to inhibit trypsin is higher than that of chymotrypsin and plasmin. In addition, it also inhibits plasma kallikrein and coagulation factor Xa, but it does not inhibit metalloproteinases, tissue kallikrein, acidic proteases, and sulfur-containing proteases. Inhibitors and proteases can be equimolarly operatively bound to form a stable complex that blocks the active site of the enzyme.The pH ranges of suitable trypsin inhibition is pH 6.0-10.0. The recombinant soybean trypsin inhibitor is expressed in recombinant E. coli and purified. No animal-derived contamination was found and no other proteases were contained.
 
2.MAIN FEATURES
(1) Appearance: white, off- white, yellowish powder, loading capacity is 1.0, 10.0mg / support (based on enzyme protein content).
(2) Special activity: ≥1.0 U/mg pro.
(3) Protein content: lable amount 90%-110%.
(4) Identify (SDS-PAGE): Single main band.
(5) Molecular weight of SDS-PAGE Identify of 20.1±2.0 kD.
(6) Purity(HPLC): Area normalization method main peak purity is not less than 90%.
Inhibitor activity unit: The ability to inhibit one trypsin unit is called an inhibitor activity unit (U).
Trypsin unit: Hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.
 
3.RECOMMEND USAGE 
Effective concentration is equimolar with protease. The recommend binding is pH>6.0 and it will dissociate at pH <5.0.
 
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant soybean trypsin Inhibitor should be stored under 2-8℃ in sealed container. It is stable within 24 months at 2-8℃.
Stability of transport: The product is stable by blue ice insulation transport.
 
5.PRODUCT ADVANTAGE
Animal origin free: Recombinant soybean trypsin Inhibitor is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents:we can provide relevant regular support files in according to customers’ requirement.
 
6.PRODUCT USAGE
Soybean trypsin inhibitor is a serine protease inhibitor that can form a stable complex with proteases to close the active site of the enzyme and can be used in place of soybean trypsin inhibitors in various biotechnological processes. Such as inhibition of serine protease activity in the production of recombinant proteins, cell culture and other biotechnology processes.
 
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Recombinant trypsin
Recombinant human trypsin
Recombinant enterokinase

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