Product•Recombinant Enzymes

R-Human Chymotrypsin
 Recombinant Human Chymotrypsin

Catalog Number:RCT10
CAS: 9004-07-3
EC: 3.4.21.1
Storage temperature: 2-8℃
Source: Recombinant human chymotrypsin is a genetically engineered protein expressed in E.coli 
Protein Sequence:The amino acid sequence of recombinant human chymotrypsin is identical to human chymotrypsin
 
1.DESCRIPTION
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
 
2.MAIN FEATURES 

Source Recombinant E. coli
Appearance White or off white powder
Specific activity ≥1000 USP units/mg pro.
SDS-PAGE Single major band
SDS-PAGE (MW) 24.0±2.4 kDa
UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.
 
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0. 
 
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
 
5.PRODUCT ADVANTAGE
Animal origin free: Recombinant human chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
 
6.PRODUCT USAGE
Used alone, or with other proteases to interact with digestive proteins, or other special uses such as organic synthesis.
 
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade chymotrypsin
Recombinant trypsin
Recombinant enterokinase
 
 
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