Product•Recombinant Enzymes

Trypsin Solution
Recombinant Trypsin Solution


DESCRIPTION
Recombinant trypsin solution, is an animalcomponent free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

FEATURES AND BENEFITS
Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination
Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.
Recombinant trypsin
Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.
High purity
Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.
High safety
The trypsin solution, readyto-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.
Convenient
Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.
Good work without EDTA
No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.

METHOD OF USE
Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.
Step 2. Dissolve the trypsin powder, and then pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.

NOTES:
Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.
Note 2. If necessary, after dissolving, immediately dispense into suitable containers, then stored under -20℃. To use it, dissolve it at room temperature.
Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.

PRODUCTS INFORMATION
Product Cat. No. Activity Packaging Manufacturer
Recombinant Trypsin Solution
RTS04
2000 BAEE u/ml
100 ml,500ml YaxinBio

UNIT DEFINITION:One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃ (1cm light path).

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Shanghai Yaxin Biotechnology Co.,Ltd

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